Unlike techniques such as ion exchange chromatography (IEX) or affinity chromatography (AC), molecules do not bind to the chromatography resin, which means that buffer composition does not directly affect resolution (the degree of separation between peaks). SEC separates molecules by differences in size as they pass through a resin packed in a column. Size exclusion chromatography (SEC), also known as gel filtration, is the mildest of all the chromatography techniques. Troubleshooting common issues when performing SEC.How to use SEC for desalting and buffer exchange.When should I use preparative SEC for protein purification?.How does size exclusion chromatography work?.By Jon Lundqvist, Senior Scientist, Cytiva
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |